{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q809S5","uniProtkbId":"NCAP_I01A0","entryAudit":{"firstPublicDate":"2007-11-13","lastAnnotationUpdateDate":"2024-05-29","lastSequenceUpdateDate":"2008-07-22","entryVersion":68,"sequenceVersion":2},"annotationScore":3.0,"organism":{"scientificName":"Influenza A virus (strain A/Silky Chicken/Hong Kong/SF189/2001 H5N1 genotype A)","taxonId":196430,"lineage":["Viruses","Riboviria","Orthornavirae","Negarnaviricota","Polyploviricotina","Insthoviricetes","Articulavirales","Orthomyxoviridae","Alphainfluenzavirus","Alphainfluenzavirus influenzae","Influenza A virus"]},"organismHosts":[{"scientificName":"Aves","taxonId":8782},{"scientificName":"Felis catus","commonName":"Cat","synonyms":["Felis silvestris catus"],"taxonId":9685},{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606},{"scientificName":"Panthera pardus","commonName":"Leopard","synonyms":["Felis pardus"],"taxonId":9691},{"scientificName":"Panthera tigris","commonName":"Tiger","taxonId":9694},{"scientificName":"Sus scrofa","commonName":"Pig","taxonId":9823}],"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04070"}],"value":"Nucleoprotein"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04070"}],"value":"Nucleocapsid protein"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04070"}],"value":"Protein N"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04070"}],"value":"NP"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04070"}],"value":"Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04070"}],"value":"Homomultimerizes to form the nucleocapsid. May bind host exportin-1/XPO1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04070"}],"value":"Virion","id":"SL-0274"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04070"}],"value":"Host nucleus","id":"SL-0414"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04070"}],"value":"Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04070"}],"value":"Belongs to the influenza viruses nucleoprotein family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":498,"modifier":"EXACT"}},"description":"Nucleoprotein","featureId":"PRO_0000310915"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Motif","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":18,"modifier":"EXACT"}},"description":"Unconventional nuclear localization signal","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04070"}]},{"type":"Motif","location":{"start":{"value":198,"modifier":"EXACT"},"end":{"value":216,"modifier":"EXACT"}},"description":"Bipartite nuclear localization signal","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04070"}]}],"keywords":[{"id":"KW-0167","category":"Cellular component","name":"Capsid protein"},{"id":"KW-1139","category":"Cellular component","name":"Helical capsid protein"},{"id":"KW-1048","category":"Cellular component","name":"Host nucleus"},{"id":"KW-0945","category":"Biological process","name":"Host-virus interaction"},{"id":"KW-0687","category":"Molecular function","name":"Ribonucleoprotein"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"id":"KW-0543","category":"Molecular function","name":"Viral nucleoprotein"},{"id":"KW-1163","category":"Biological process","name":"Viral penetration into host nucleus"},{"id":"KW-0946","category":"Cellular component","name":"Virion"},{"id":"KW-1160","category":"Biological process","name":"Virus entry into host cell"}],"references":[{"referenceNumber":1,"citation":{"id":"12077307","citationType":"journal article","authors":["Guan Y.","Peiris J.S.M.","Lipatov A.S.","Ellis T.M.","Dyrting K.C.","Krauss S.","Zhang L.J.","Webster R.G.","Shortridge K.F."],"citationCrossReferences":[{"database":"PubMed","id":"12077307"},{"database":"DOI","id":"10.1073/pnas.132268999"}],"title":"Emergence of multiple genotypes of H5N1 avian influenza viruses in Hong Kong SAR.","publicationDate":"2002","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"8950","lastPage":"8955","volume":"99"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC RNA]"]},{"referenceNumber":2,"citation":{"id":"CI-2KPPGU9LDC52R","citationType":"submission","authors":["Li K.S.","Xu K.M.","Guan Y."],"publicationDate":"MAR-2008","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["SEQUENCE REVISION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF509122","properties":[{"key":"ProteinId","value":"AAO52965.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"SMR","id":"Q809S5","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0019029","properties":[{"key":"GoTerm","value":"C:helical viral capsid"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0043657","properties":[{"key":"GoTerm","value":"C:host cell"},{"key":"GoEvidenceType","value":"IEA:GOC"}]},{"database":"GO","id":"GO:0042025","properties":[{"key":"GoTerm","value":"C:host cell nucleus"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:1990904","properties":[{"key":"GoTerm","value":"C:ribonucleoprotein complex"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0019013","properties":[{"key":"GoTerm","value":"C:viral nucleocapsid"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003723","properties":[{"key":"GoTerm","value":"F:RNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0005198","properties":[{"key":"GoTerm","value":"F:structural molecule activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0046718","properties":[{"key":"GoTerm","value":"P:symbiont entry into host cell"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0075732","properties":[{"key":"GoTerm","value":"P:viral penetration into host nucleus"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"HAMAP","id":"MF_04070","properties":[{"key":"EntryName","value":"INFV_NCAP"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002141","properties":[{"key":"EntryName","value":"Flu_NP"}]},{"database":"Pfam","id":"PF00506","properties":[{"key":"EntryName","value":"Flu_NP"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF161003","properties":[{"key":"EntryName","value":"flu NP-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MASQGTKRSYEQMETGGERQNATEIRASVGRMVGGIGRFYIQMCTELKLSDYEGRLIQNSITMERMVLSAFDERRNKYLEEHPSAGKDPKKTGGPIYRRRDGKWVRELILYDKEEIRRIWRQANNGEDATAGLTHLMIWHSNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELIRMIKRGINDRNFWRGENGRRTRIAYERMCNILKGKFQTAAQRAMMDQVRESRNPGNAEIEDLIFLARSALILRGSVAHKSCLPACVYGLAVASGYDFEREGYSLVGIDPFRLLQNSQVFSLIRPNENPAHKSQLVWMACHSAAFEDLRVSSFIRGTRIVPRGQLSTRGVQIASNENMDTMDSNTLELRSRYWAIRTRSGGNTNQQRASAGQISVQPTFSVQRNLPFERATIMAAFTGNTEGRTSDMRTEIIRMMESAKPEDVSFQGRGVFELSDEKATNPIVPSFDMSNEGSYFFGDNAEEFEN","length":498,"molWeight":56267,"crc64":"8EDE53F37FD0950F","md5":"9F057FE9EED5A08FDBA3FA160014FDE0"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"PTM":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":1,"Motif":2},"uniParcId":"UPI00017172C1"}}