{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"G2WWV6","secondaryAccessions":["G0Y276","P86840"],"uniProtkbId":"PEVD1_VERDV","entryAudit":{"firstPublicDate":"2020-10-07","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2011-11-16","entryVersion":36,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Verticillium dahliae (strain VdLs.17 / ATCC MYA-4575 / FGSC 10137)","commonName":"Verticillium wilt","taxonId":498257,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Sordariomycetes","Hypocreomycetidae","Glomerellales","Plectosphaerellaceae","Verticillium"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"21691787"}],"value":"Hypersensitive response-inducing protein PevD1"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"33186613"}],"value":"Effector protein PevD1"}}],"flag":"Precursor"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"21691787"}],"value":"PevD1"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"EGY21211.1"}],"value":"VDAG_02735"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21691787"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24080193"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24337817"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27489497"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29563924"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30295911"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33186613"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34303024"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36342142"}],"value":"Effector protein that elicits a hypersensitive response (HR) in tobacco plants (Nicotiana tabacum) and cotton (Gossypium hirsutum) (PubMed:21691787, PubMed:24080193, PubMed:24337817). Boosts systemic acquired resistance (SAR) to tobacco mosaic virus (TMV) infection in Nicotiana tabacum and to Verticillium dhaliae infection in primed cotton seedlings (PubMed:21691787, PubMed:24080193, PubMed:24337817, PubMed:29563924). PevD1 induces the expression of genes related to the phenylpropanoid metabolic pathway and enhances lignin deposition and vessel reinforcement in cotton (PubMed:24337817). Increases slightly jasmonate (JA) levels in Arabidopsis and induces the expression of repressors of the abscisic acid (ABA)-responsive pathway to suppress the ABA signal transduction (PubMed:27489497). Triggers also the Ca(2+)-responsive pathways in Arabidopsis (PubMed:27489497). In cotton, PevD1 binds to the pathogenesis-related protein 5 (PR5) to inhibit its antifungal activity in order to overcome the host defense system (PubMed:30295911). In Nicotiana benthamiana, PevD1 interacts with the asparagine-rich protein NRP1 to mediate PevD1-induced defense responses by regulating sesquiterpenoid phytoalexins capsidiol biosynthesis pathway (PubMed:29563924, PubMed:33186613). In both Arabidopsis and cotton, PevD1 interacts with and stabilizes the senescence-associated transcription factor ORE1 by disrupting its interaction with the RING-type ubiquitin E3 ligase NLA. ORE1 directly binds the promoter of ACS6 and promotes its expression for mediating PevD1-induced ethylene biosynthesis and subsequent leaf senescence (PubMed:34303024). In Arabidopsis thaliana, PevD1 strongly induces the ethylene-responsive factor ERF114 to positively modulate disease resistance by targeting the promoter of PAL1 and activating PAL1 transcription, resulting in an increase in lignin and salicylic acid (SA) levels (PubMed:36342142)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22750869"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28633330"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29563924"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30295911"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33186613"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34303024"}],"value":"Monomer (PubMed:22750869). Interacts with Arabidopsis thaliana and Nicotiana benthamiana asparagine-rich proteins (NRPs) (PubMed:28633330, PubMed:29563924, PubMed:33186613). Interacts with Gossypium hirsutum pathogenesis-related protein 5 (PR5) (PubMed:30295911). Interacts also with Arabidopsis thaliana ORE1 (PubMed:34303024)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21691787"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30295911"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36342142"}],"value":"Expression is induced throughout the plant infection process (PubMed:30295911). Expression is positively regulated by the C2H2-type zinc finger transcription factor CF2 (PubMed:36342142)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24080193"}],"value":"The N-terminal region (1-98) is sufficient for induction of SAR of N.tabacum to TMV infection. The C-terminal region (99-155) is sufficient for eliciting HR in N.tabacum"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30295911"}],"value":"Displays attenuated pathogenicity"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":18,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":19,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"Hypersensitive response-inducing protein PevD1","featureId":"PRO_0000451206"},{"type":"Domain","location":{"start":{"value":33,"modifier":"EXACT"},"end":{"value":148,"modifier":"EXACT"}},"description":"AA1-like","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01243"}]},{"type":"Disulfide bond","location":{"start":{"value":70,"modifier":"EXACT"},"end":{"value":84,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01243"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28633330"}]},{"type":"Disulfide bond","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01243"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28633330"}]},{"type":"Sequence conflict","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"in Ref. 1; AA sequence","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["Q"]}},{"type":"Sequence conflict","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"in Ref. 1; AA sequence","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"SGD","alternativeSequences":["VDAS"]}}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0732","category":"Domain","name":"Signal"}],"references":[{"referenceNumber":1,"citation":{"id":"21691787","citationType":"journal article","authors":["Wang B.","Yang X.","Zeng H.","Liu H.","Zhou T.","Tan B.","Yuan J.","Guo L.","Qiu D."],"citationCrossReferences":[{"database":"PubMed","id":"21691787"},{"database":"DOI","id":"10.1007/s00253-011-3405-1"}],"title":"The purification and characterization of a novel hypersensitive-like response-inducing elicitor from Verticillium dahliae that induces resistance responses in tobacco.","publicationDate":"2012","journal":"Appl. Microbiol. Biotechnol.","firstPage":"191","lastPage":"201","volume":"93"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","PROTEIN SEQUENCE OF 28-88","FUNCTION","SUBCELLULAR LOCATION"]},{"referenceNumber":2,"citation":{"id":"21829347","citationType":"journal article","authors":["Klosterman S.J.","Subbarao K.V.","Kang S.","Veronese P.","Gold S.E.","Thomma B.P.H.J.","Chen Z.","Henrissat B.","Lee Y.-H.","Park J.","Garcia-Pedrajas M.D.","Barbara D.J.","Anchieta A.","de Jonge R.","Santhanam P.","Maruthachalam K.","Atallah Z.","Amyotte S.G.","Paz Z.","Inderbitzin P.","Hayes R.J.","Heiman D.I.","Young S.","Zeng Q.","Engels R.","Galagan J.","Cuomo C.A.","Dobinson K.F.","Ma L.-J."],"citationCrossReferences":[{"database":"PubMed","id":"21829347"},{"database":"DOI","id":"10.1371/journal.ppat.1002137"}],"title":"Comparative genomics yields insights into niche adaptation of plant vascular wilt pathogens.","publicationDate":"2011","journal":"PLoS Pathog.","firstPage":"E1002137","lastPage":"E1002137","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"VdLs.17 / ATCC MYA-4575 / FGSC 10137","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"24080193","citationType":"journal article","authors":["Liu W.","Zeng H.","Liu Z.","Yang X.","Guo L.","Qiu D."],"citationCrossReferences":[{"database":"PubMed","id":"24080193"},{"database":"DOI","id":"10.1016/j.micres.2013.08.001"}],"title":"Mutational analysis of the Verticillium dahliae protein elicitor PevD1 identifies distinctive regions responsible for hypersensitive response and systemic acquired resistance in tobacco.","publicationDate":"2014","journal":"Microbiol. Res.","firstPage":"476","lastPage":"482","volume":"169"},"referencePositions":["FUNCTION","DOMAIN"]},{"referenceNumber":4,"citation":{"id":"24337817","citationType":"journal article","authors":["Bu B.","Qiu D.","Zeng H.","Guo L.","Yuan J.","Yang X."],"citationCrossReferences":[{"database":"PubMed","id":"24337817"},{"database":"DOI","id":"10.1007/s00299-013-1546-7"}],"title":"A fungal protein elicitor PevD1 induces Verticillium wilt resistance in cotton.","publicationDate":"2014","journal":"Plant Cell Rep.","firstPage":"461","lastPage":"470","volume":"33"},"referencePositions":["FUNCTION"]},{"referenceNumber":5,"citation":{"id":"27489497","citationType":"journal article","authors":["Liu M.","Khan N.U.","Wang N.","Yang X.","Qiu D."],"citationCrossReferences":[{"database":"PubMed","id":"27489497"},{"database":"DOI","id":"10.7150/ijbs.15447"}],"title":"The Protein Elicitor PevD1 Enhances Resistance to Pathogens and Promotes Growth in Arabidopsis.","publicationDate":"2016","journal":"Int. J. Biol. Sci.","firstPage":"931","lastPage":"943","volume":"12"},"referencePositions":["FUNCTION"]},{"referenceNumber":6,"citation":{"id":"29563924","citationType":"journal article","authors":["Liang Y.","Cui S.","Tang X.","Zhang Y.","Qiu D.","Zeng H.","Guo L.","Yuan J.","Yang X."],"citationCrossReferences":[{"database":"PubMed","id":"29563924"},{"database":"DOI","id":"10.3389/fpls.2018.00303"}],"title":"An Asparagine-Rich Protein Nbnrp1 Modulate Verticillium dahliae Protein PevD1-Induced Cell Death and Disease Resistance in Nicotiana benthamiana.","publicationDate":"2018","journal":"Front. Plant Sci.","firstPage":"303","lastPage":"303","volume":"9"},"referencePositions":["FUNCTION","INTERACTION WITH NICOTIANA BENTHAMIANA NRP1"]},{"referenceNumber":7,"citation":{"id":"30295911","citationType":"journal article","authors":["Zhang Y.","Gao Y.","Liang Y.","Dong Y.","Yang X.","Qiu D."],"citationCrossReferences":[{"database":"PubMed","id":"30295911"},{"database":"DOI","id":"10.1093/jxb/ery351"}],"title":"Verticillium dahliae PevD1, an Alt a 1-like protein, targets cotton PR5-like protein and promotes fungal infection.","publicationDate":"2019","journal":"J. Exp. Bot.","firstPage":"613","lastPage":"626","volume":"70"},"referencePositions":["INDUCTION","FUNCTION","DISRUPTION PHENOTYPE","INTERACTION WITH GOSSYPIUM HIRSUTUM PR5"]},{"referenceNumber":8,"citation":{"id":"33186613","citationType":"journal article","authors":["Liang Y.","Li Z.","Zhang Y.","Meng F.","Qiu D.","Zeng H.","Li G.","Yang X."],"citationCrossReferences":[{"database":"PubMed","id":"33186613"},{"database":"DOI","id":"10.1016/j.gene.2020.145280"}],"title":"Nbnrp1 mediates Verticillium dahliae effector PevD1-triggered defense responses by regulating sesquiterpenoid phytoalexins biosynthesis pathway in Nicotiana benthamiana.","publicationDate":"2021","journal":"Gene","firstPage":"145280","lastPage":"145280","volume":"768"},"referencePositions":["FUNCTION","INTERACTION WITH NICOTIANA BENTHAMIANA NRP1"]},{"referenceNumber":9,"citation":{"id":"34303024","citationType":"journal article","authors":["Zhang Y.","Gao Y.","Wang H.L.","Kan C.","Li Z.","Yang X.","Yin W.","Xia X.","Nam H.G.","Li Z.","Guo H."],"citationCrossReferences":[{"database":"PubMed","id":"34303024"},{"database":"DOI","id":"10.1016/j.molp.2021.07.014"}],"title":"Verticillium dahliae secretory effector PevD1 induces leaf senescence by promoting ORE1-mediated ethylene biosynthesis.","publicationDate":"2021","journal":"Mol. Plant","firstPage":"1901","lastPage":"1917","volume":"14"},"referencePositions":["FUNCTION","INTERACTION WITH PLANT ORE1"]},{"referenceNumber":10,"citation":{"id":"36342142","citationType":"journal article","authors":["Liu T.","Qin J.","Cao Y.","Subbarao K.V.","Chen J.","Mandal M.K.","Xu X.","Shang W.","Hu X."],"citationCrossReferences":[{"database":"PubMed","id":"36342142"},{"database":"DOI","id":"10.1128/aem.01385-22"}],"title":"Transcription Factor VdCf2 Regulates Growth, Pathogenicity, and the Expression of a Putative Secondary Metabolism Gene Cluster in Verticillium dahliae.","publicationDate":"2022","journal":"Appl. Environ. Microbiol.","firstPage":"e0138522","lastPage":"e0138522","volume":"88"},"referencePositions":["INDUCTION"]},{"referenceNumber":11,"citation":{"id":"35340106","citationType":"journal article","authors":["Li Z.","Zhang Y.","Ren J.","Jia F.","Zeng H.","Li G.","Yang X."],"citationCrossReferences":[{"database":"PubMed","id":"35340106"},{"database":"DOI","id":"10.1111/mpp.13208"}],"title":"Ethylene-responsive factor ERF114 mediates fungal pathogen effector PevD1-induced disease resistance in Arabidopsis thaliana.","publicationDate":"2022","journal":"Mol. Plant Pathol.","firstPage":"819","lastPage":"831","volume":"23"},"referencePositions":["FUNCTION"]},{"referenceNumber":12,"citation":{"id":"22750869","citationType":"journal article","authors":["Han L.","Liu Z.","Liu X.","Qiu D."],"citationCrossReferences":[{"database":"PubMed","id":"22750869"},{"database":"DOI","id":"10.1107/s1744309112020556"}],"title":"Purification, crystallization and preliminary X-ray diffraction analysis of the effector protein PevD1 from Verticillium dahliae.","publicationDate":"2012","journal":"Acta Crystallogr. F","firstPage":"802","lastPage":"805","volume":"68"},"referencePositions":["CRYSTALLIZATION","PRELIMINARY X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS)","SUBUNIT"]},{"referenceNumber":13,"citation":{"id":"28633330","citationType":"journal article","authors":["Zhou R.","Zhu T.","Han L.","Liu M.","Xu M.","Liu Y.","Han D.","Qiu D.","Gong Q.","Liu X."],"citationCrossReferences":[{"database":"PubMed","id":"28633330"},{"database":"DOI","id":"10.1093/jxb/erx192"}],"title":"The asparagine-rich protein NRP interacts with the Verticillium effector PevD1 and regulates the subcellular localization of cryptochrome 2.","publicationDate":"2017","journal":"J. Exp. Bot.","firstPage":"3427","lastPage":"3440","volume":"68"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS)","DISULFIDE BONDS","INTERACTION WITH ARABIDOPSIS THALIANA NRP"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"HQ540585","properties":[{"key":"ProteinId","value":"ADW79419.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"DS572698","properties":[{"key":"ProteinId","value":"EGY21211.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"XP_009651683.1","properties":[{"key":"NucleotideSequenceId","value":"XM_009653388.1"}]},{"database":"PDB","id":"5XMZ","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.85 A"},{"key":"Chains","value":"A=1-155"}]},{"database":"PDBsum","id":"5XMZ","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"G2WWV6","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"G2WWV6","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"498257.G2WWV6","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"EGY21211","properties":[{"key":"ProteinId","value":"EGY21211"},{"key":"GeneId","value":"VDAG_02735"}]},{"database":"GeneID","id":"20704198","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"vda:VDAG_02735","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG502SP40","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_144932_1_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"G2WWV6","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"GPADEIC","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"23786at1028384","properties":[{"key":"Description","value":"-"}]},{"database":"PHI-base","id":"PHI:8520","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001611","properties":[{"key":"Component","value":"Chromosome 3"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"CDD","id":"cd12798","properties":[{"key":"EntryName","value":"Alt_A1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.350.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR032382","properties":[{"key":"EntryName","value":"AltA1"}]},{"database":"Pfam","id":"PF16541","properties":[{"key":"EntryName","value":"AltA1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51895","properties":[{"key":"EntryName","value":"AA1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MQFTLAAAAALFGASALAAPASPGSTGAPPDPNMYENIDIADFNVRKGEDGTIKYVNFKLSGDDADGLLCEAQNPGLPSNVITCGESKYRFALSSGKQYEFALSLYHELGLAVGFYGTGEIFTHCRAGGLGDFICQQQNPTTIVIDSLPDAPAEA","length":155,"molWeight":16230,"crc64":"230EA4200C0296BF","md5":"4D1A99AF12FF420DD98D0D24BED52CF1"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DOMAIN":1,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Disulfide bond":2,"Sequence conflict":2},"uniParcId":"UPI000218FF65"}}